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Customised flow cytometry solutions

Engage assay experts with extensive experience and expertise

Full service assay development

 

ICON provides industry-recognised research and development services to support technology transfers, assay development and validation to monitor key molecules, targets and receptors. ICON has the resources and scientific expertise to implement a broad range of custom flow cytometric assays in clinical trials.

 

ICON works with sponsors to:

  • Develop and/or optimise flow cytometry methodology to meet the required specifications for monitoring therapeutics
  • Provide the sponsor with validation data and work in a collaborative manner during the assay development/validation stage
  • Roll out flow cytometric assays globally as required
  • Develop a customised standard operating procedure (SOP) for performing assays globally
  • Collaborate with the sponsor regarding special requirements, including secure transfer of listmode and data analysis files
  • Utilise mature operational processes that optimise the sponsor’s requirements
  • Precisely define the specifications for result reporting

We have expertise in using a variety of instrumentation platforms, and developing & validating a broad range of complex flow cytometric assays under rigorous quality control procedures (see factsheet for details). Dedicated operational staff perform ongoing clinical trial sample processing and analysis using standardised SOPs and data analysis strategies.

Global flow cytometry network

Our global flow cytometry network has laboratory locations in New York, Dublin, Singapore, and Beijing in the cental laboratory setting and in Lenexa and Assen in the bioanalytical laboratory setting and all instruments are connected to a central flow cytometry server. Having a central server allows us to utilise fully harmonised and standardised processes and instrumentation for even the most complex assays. This network enables mature processes for developing, validating and implementing complex flow cytometric assays globally.  We offer up to 25 color flow cytometry globally, including North America, Europe, and Asia.

Complex flow cytometry assays

Examples of complex flow cytometry assays validated at ICON include:

  • Receptor occupancy assays customised per study drug
  • T cell and B cell subset immunophenotyping along with monocyte, granulocyte, NK, and basophil identification
  • Rare event cell enumeration including detection of tetramer labelled antigen specific lymphocytes, myeloid derived suppressor cells, dendritic cell subpopulations and blasts 
  • Functional assays such as in vitro stimulation and quantification of intracellular cytokines and phosphorylation events
  • Intracellular small molecule measurement, including measurement of antibody-drug-conjugates correlating to a relative amount of drug delivered per cell
  • MACS bead separation assays
  • Antibody-Dependent Cell-mediated Cytotoxicity (ADCC) assays
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